PRESENILIN-1 (N-TERM); RT X-100UL

Code: MAB1563 D2-231

Analysis Note

ControlBrain tissue

Application

Immunohistochemistry: rat pups and 24 old month rats were perfused for 10 minutes with 100-200mL of...


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Analysis Note

ControlBrain tissue

Application

Immunohistochemistry: rat pups and 24 old month rats were perfused for 10 minutes with 100-200mL of 4% PFA 0.3% glutaraldehyde and 0.1% CaCl2 in 0.1M PBS pH 7.4. Vibratome sections were blocked with 3% normal serum and permeabilized with 0.1% triton X-100.{Neuroscience 2003 120:405-423}.

Immunoblotting: 1:250-1:500. Recommend blocking buffer is TBS containing 5% non fat milk and 0.01% Tween 20. It is recommended that you also dilute the antibody in this blocking buffer. Incubate with the MAB1563 for 1 to 2 hours at room temperature or overnight at 4°C.

Immunoprecipitation

ELISA

Optimal working dilutions must be determined by end user.

Research Sub CategoryNeurodegenerative Diseases

Research CategoryNeuroscience

This Anti-Presenilin-1 Antibody, N-terminus, clone hPS1-NT is validated for use in ELISA, IH, IP, WB for the detection of Presenilin-1.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

A fusion protein antigen containing the N-terminus of human PS-1 (residues 21-80) fused to GST.

Epitope: N-terminus

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

UnPurified mouse tissue culture supernatant from a perfusion system, filtered through a 0.2µ micron membrane prior to vialing. Product contains 20ûS and Ciprofloxacin at final concentration of 10µg/mL.

Unpurified

Specificity

Recognizes Presenilin-1, human. By Western blot the antibody recognizes a predominant 32 kDa polypeptide in a variety of samples, including PC12 cells transfected with human PS1 complementary DNA, brain biopsy specimens from demented patients, and postmortem samples of frontal neucortex from Familial Alzheimer′s Disease cases, late-onset Alzheimer′s disease cases, and cases of other degenerative disorders. Immunohistochemical studies of control brains revealed that PS1 is expressed primarily in neurons, with the protein localizing in the soma and dendritic processes. In contrast in FAD and sporadic Alzheimer′s disease cases, PS1 immunoreactivity was present in the neuritic component of senile plaques as well as in neurofibrillary tangles. Localization of PS1 immunoreactivity in familial and sporadic Alzheimer′s disease suggest that genetically heterogeneous forms of the disease share a common pathophysiology involving PS1 protein.

Storage and Stability

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Target description

32 kDa

antibody formculture supernatant
antibody product typeprimary antibodies
biological sourcerat
clonehPS1-NT, monoclonal
Gene Informationhuman ... PSEN1(5663)
isotypeIgG2a
manufacturer/tradenameChemicon®
NCBI accession no.NM_000021.2
Quality Level100
shipped inwet ice
species reactivityhuman
technique(s)western blot: suitable, immunoprecipitation (IP): suitable, ELISA: suitable, immunohistochemistry: suitable
UniProt accession no.P49768
This product has met the following criteria to qualify for the following awards:



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